In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
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- CAS-Nr.
- Englisch Name:
- In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
- Synonyma:
- In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
- CBNumber:
- CB92096244
- Summenformel:
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
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In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Eigenschaften
Sicherheit
- Risiko- und Sicherheitserklärung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
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| Alarmwort |
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| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H412 |
Schädlich für Wasserorganismen, mit langfristiger Wirkung. |
Langfristig (chronisch) gewässergefährdend |
Kategorie 3 |
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P273, P501 |
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| Sicherheit |
| P273 |
Freisetzung in die Umwelt vermeiden. |
| P501 |
Inhalt/Behälter ... (Entsorgungsvorschriften vom Hersteller anzugeben) zuführen. |
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In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Chemische Eigenschaften,Einsatz,Produktion Methoden
R-Sätze Betriebsanweisung:
R22:Gesundheitsschädlich beim Verschlucken.
R41:Gefahr ernster Augenschäden.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Verwenden
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:
- to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
- to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
- to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
- for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.
Allgemeine Beschreibung
Tox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Biochem/physiol Actions
The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative..
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 3)Lieferanten
- In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based
- Cell AnalysisCell Signaling and Neuroscience
- Cell Biology
- Cell Growth and Cell Viability KitsReagents and Supplements
- Cell Viability and Proliferation
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