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ALPHA-KETOGLUTARATE DEHYDROGENASE

CAS No.
9031-02-1
Chemical Name:
ALPHA-KETOGLUTARATE DEHYDROGENASE
Synonyms
α-Ketoglutarate Dehydrogenase;ALPHA-KETOGLUTARATE DEHYDROGENASE;α-ketoglutarate dehydrogenase from porcine heart;multienzyme 2-oxoglutarate dehydrogenase complex;A-ketoglutarate dehydrogenase from*porcine heart;α-Ketoglutarate Dehydrogenase, from porcine heart;alpha-Ketoglutarate Dehydrogenase from porcine heart;MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2
CBNumber:
CB0239707
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00130490
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

ALPHA-KETOGLUTARATE DEHYDROGENASE Properties

storage temp. -20°C
form buffered aqueous glycerol solution

SAFETY

Risk and Safety Statements

Hazard statements  H412
Precautionary statements  P273-P501
WGK Germany  3

ALPHA-KETOGLUTARATE DEHYDROGENASE price More Price(3)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich K1502 α-Ketoglutarate Dehydrogenase from porcine heart buffered aqueous glycerol solution, 0.1-1.0?units/mg protein (Lowry) 9031-02-1 20UNITS $233 2023-06-20 Buy
Sigma-Aldrich K1502 α-Ketoglutarate Dehydrogenase from porcine heart buffered aqueous glycerol solution, 0.1-1.0?units/mg protein (Lowry) 9031-02-1 50UNITS $483 2023-06-20 Buy
American Custom Chemicals Corporation ENZ0000645 ALPHA-KETOGLUTARATE DEHYDROGENASE-PORCINE HEART 95.00% 9031-02-1 5MG $611.85 2021-12-16 Buy
Product number Packaging Price Buy
K1502 20UNITS $233 Buy
K1502 50UNITS $483 Buy
ENZ0000645 5MG $611.85 Buy

ALPHA-KETOGLUTARATE DEHYDROGENASE Chemical Properties,Uses,Production

Uses

α-Ketoglutarate Dehydrogenase from the porcine heart has been used:

  • to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
  • to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
  • as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

General Description

α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

Biochem/physiol Actions

α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

ALPHA-KETOGLUTARATE DEHYDROGENASE Preparation Products And Raw materials

Raw materials

Preparation Products

ALPHA-KETOGLUTARATE DEHYDROGENASE Suppliers

Global( 7)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Guangzhou Tomums Life Science Co., Ltd. 020-31155029 18902330969 sales@tomums.cn China 4698 58
Shanghai Guchen Biotechnology Co., LTD 021-34675735 19147740836 1986399151@qq.com China 9849 58
ALPHA-KETOGLUTARATE DEHYDROGENASE A-ketoglutarate dehydrogenase from*porcine heart multienzyme 2-oxoglutarate dehydrogenase complex α-ketoglutarate dehydrogenase from porcine heart MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2 α-Ketoglutarate Dehydrogenase alpha-Ketoglutarate Dehydrogenase from porcine heart α-Ketoglutarate Dehydrogenase, from porcine heart 9031-02-1 BioChemical General Metabolic Enzymes I-Z General Metabolic Enzymes Metabolomics