Tris-Glycine Buffer 10× Concentrate
- CAS No.
- Chemical Name:
- Tris-Glycine Buffer 10× Concentrate
- Synonyms
- Tris-Glycine Buffer 10× Concentrate ;Tris-Glycine Buffer 10x Concentrate Vetec(TM) reagent grade, 10 x, solution, protease free
- CBNumber:
- CB02920885
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Tris-Glycine Buffer 10× Concentrate Chemical Properties,Uses,Production
Uses
Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.
Biochem/physiol Actions
For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
Tris-Glycine Buffer 10× Concentrate Preparation Products And Raw materials
Raw materials
Preparation Products
Tris-Glycine Buffer 10× Concentrate Suppliers
Global( 2)Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Supplier | Advantage |
|---|---|
| Sigma-Aldrich | 80 |
Tris-Glycine Buffer 10× Concentrate
Tris-Glycine Buffer 10x Concentrate Vetec(TM) reagent grade, 10 x, solution, protease free