Anti-Phosphotyrosine antibody, Mouse monoclonal

Uses

Monoclonal Anti-Phosphotyrosine has been used in

• immunoblotting,
• immunofluorescence,
• immunohistochemistry
• immunocytochemistry
• flow cytometry
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)
• radio immunoassay (RIA)
• immunoaffinity isolation

Uses

Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• western blot
• immunohistochemistry

General Description

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.

Biochem/physiol Actions

Protein phosphorylation is a basic signaling mechanism that modifies protein function in eukaryotic cells. Serine, threonine, and tyrosine are the major phosphorylated amino acids in proteins. Tyrosine phosphorylation is a rare post-translational event in normal tissues, accounting for only 0.03% of phosphorylated amino acids. However, this phosphorylation increases several folds by various activation signals and the process is mediated by protein tyrosine kinases. Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis, and development.