Uses
Reverse transcription is a hallmark of molecular biology. The ability to convert RNA into DNA for qualitative and quantitative RNA analysis using PCR or real-time PCR has revolutionized gene expression analysis. Quantitative reverse transcription polymerase chain reaction (RT-PCR or qRT-PCR) is now the standard for mRNA detection and quantification, sensitive enough to quantify RNA from a single cell.
RT-PCR is also used for the creation of cDNA libraries, molecular cloning by PCR, and sequencing. The Transcriptor Universal cDNA Master is a convenient fast solution for cDNA synthesis and real-time PCR analysis. No matter which PCR instrument you are using, this master mix, supplied in just two vials, brings out the full potential of your qRT-PCR assays.
General Description
Ready-to-use reaction mix for two-step RT-qPCR on all real-time PCR systems.
The Transcriptor Universal cDNA Master is the convenient solution for time-saving cDNA synthesis for two-step real-time RT-PCR. All reagents required, including random hexamer primer, nucleotides, buffers and enzymes are supplied in just two vials minimizing pipetting. Maximum convenience is achieved by combining the Transcriptor Universal cDNA Master mix with the RealTime ready Cell Lysis Kit, further accelerating real-time PCR by directly using cell lysates. The unique enzyme included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The Transcriptor Universal cDNA Master produces very high specificity and sensitivity, even with difficult complex RNA templates. Excellent performance and linear response in real-time RT-PCR is obtained for both low and high copy genes. The Transcriptor Universal cDNA Master is compatible with LightCycler? instruments and all other real-time PCR instruments requiring normalization with a reference dye.
