ANTI-FLAGantibody, Rat monoclonal
- CAS No.
- Chemical Name:
- ANTI-FLAGantibody, Rat monoclonal
- Synonyms
- ANTI-FLAGantibody, Rat monoclonal
- CBNumber:
- CB14669224
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
ANTI-FLAGantibody, Rat monoclonal Chemical Properties,Uses,Production
Uses
ANTI-FLAG? antibody, Rat monoclonal has been used in:
- chromatin immunoprecipitation (ChIP)
- western blotting
- coimmunoprecipitation
- flow cytometric analysis
General Description
Monoclonal Anti-FLAG? (rat IgG1 isotype) is derived from the hybridoma 6F7 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG? peptide. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Monoclonal Anti-FLAG? recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG?-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG? peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags. The small size of the FLAG? tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG? peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG? peptide from a fusion protein catalyzed by Cu2+ ions has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase, as well as in the human and bovine enzyme.
ANTI-FLAGantibody, Rat monoclonal Preparation Products And Raw materials
Raw materials
Preparation Products
ANTI-FLAGantibody, Rat monoclonal Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
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Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
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Sigma-Aldrich | 80 |