Anti-IDH1 (R132H) antibody, Mouse monoclonal
- CAS No.
- Chemical Name:
- Anti-IDH1 (R132H) antibody, Mouse monoclonal
- Synonyms
- Anti-IDH1 (R132H) antibody, Mouse monoclonal
- CBNumber:
- CB14669350
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Anti-IDH1 (R132H) antibody, Mouse monoclonal price More Price(1)
Anti-IDH1 (R132H) antibody, Mouse monoclonal Chemical Properties,Uses,Production
Uses
Anti-IDH1 (R132H) antibody has been used:
- in immunoblotting
- in immunohistochemistry
- in western blotting
- in cell cycle analysis and apoptosis assays
- in chromatin immunoprecipitation (ChIP) assay
- in in vitro migration assay
General Description
Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm.
Biochem/physiol Actions
Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas.
Anti-IDH1 (R132H) antibody, Mouse monoclonal Preparation Products And Raw materials
Raw materials
Preparation Products
Anti-IDH1 (R132H) antibody, Mouse monoclonal Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
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Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
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Sigma-Aldrich | 80 |