Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal

CAS No.
Chemical Name:
Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal
Synonyms
Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal
CBNumber:
CB14669552
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal Properties

storage temp. -20°C
form buffered aqueous solution

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D4941 Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal clone DKPS308, purified from hybridoma cell culture 200μL $433.2 2024-03-01 Buy
Sigma-Aldrich D4941 Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal clone DKPS308, purified from hybridoma cell culture 100μL $668 2024-03-01 Buy
Product number Packaging Price Buy
D4941 200μL $433.2 Buy
D4941 100μL $668 Buy

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal Chemical Properties,Uses,Production

Uses

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in:

  • western blot assay
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation

General Description

Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active.

Biochem/physiol Actions

Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser308 on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray.

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal Preparation Products And Raw materials

Raw materials

Preparation Products

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal Suppliers

Global( 1)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal