Anti-α-TubulinFITC antibody, Mouse monoclonal

Uses

Monoclonal Anti-α-Tubulin antibody is suitable for immunostaining of:

• respiratory epithelium tissue in a study to determine the tubulin expression in the mice cilia
• breast cancer tissue sections to study the effect of LMO4 on the centrosome amplification and mitotic spindle abnormalities
• spindle and chromosomes of oocytes

• detection of tubulin by immunofluorescence and confocal microscopy in lung carcinoma cell.
• immunofluorescent staining of microtubules in human embryos and mitotic spindles from spleen lymphoblast.

General Description

α/β-Tubulin, the major building block of microtubules, belongs to the tubulin superfamily of proteins. They are ubiquitously found in all eukaryotic cells. It exists in multiple isotype in many organisms.? It is a heterodimer with intracellular, hollow, cylindrical, filamentous structures. It is located on human chromosome 2q35. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β--Tubulin is a heterodimer, which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. Tubulin is a highly conserved protein with two terminal domains: large N and C terminal.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC.

Biochem/physiol Actions

The N-terminal domain of α?tubulin binds GTP, which is non-exchangeable mechanism, whereas highly acidic C-terminal domain is capable to bind microtubule-associated proteins (MAPs). Both domains are associated with the microtubular self-assembly. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. Mutations in tubulin α-4A gene leads to a neurodegenerative disease called amyotrophic lateral sclerosis.