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PCR NUCLEOTIDE MIX

PCR NUCLEOTIDE MIX Structure
PCR NUCLEOTIDE MIX structure
CAS No.
Chemical Name:
PCR NUCLEOTIDE MIX
Synonyms
DNTP/DUTP MIX;PCR NUCLEOTIDE MIX;PCR NUCLEOTIDE MIXPLUS;ADVANTAGE PCR DEOXYNUCLEOTIDE MIX
CBNumber:
CB2373124
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD01324165
MOL File:
Mol file
Last updated:2023-04-23 13:52:06
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PCR NUCLEOTIDE MIX Properties

storage temp. -20°C
form solution

PCR NUCLEOTIDE MIX price More Price(1)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
American Custom Chemicals Corporation NUC0005690 ADVANTAGE PCR DEOXYNUCLEOTIDE MIX 95.00% 5MG $496.72 2021-12-16 Buy
Product number Packaging Price Buy
NUC0005690 5MG $496.72 Buy

PCR NUCLEOTIDE MIX Chemical Properties,Uses,Production

Uses

  • This ready-to-use nucleotide mix is a premixed solution of the sodium salts of dATP, dGTP, and dCTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in water. This mix is optimized for use in all types of amplification reactions: PCR
  • RT-PCR
  • Prevention of carryover contamination
To allow decontamination of PCR or RT-PCR, dUTP in place of dTTP is incorporated into the PCR product. Subsequent reactions may then be treated with Uracil-DNA Glycosylase (UNG). Avoiding the need of re-opening the reaction vial, the vials are incubated at +20 °C, resulting in the degradation of potentially contaminating uracil-containing amplification products. During this step, template DNA and RNA remain unaffected, since normal DNA does not contain uracil, and RNA does not serve as a substrate for UNG. Before starting the actual thermocycling program, UNG is inactivated by incubation at +95 °C. Uracil -DNA Glycosylase, heat-labile is particularily useful, as it is fully inactivated already after incubation at +95 °C for 2 minutes. The natural enzyme from E. coli requires incubating the reaction mixture for 10 minutes at +95 °C. The shorter heat treatment substantially reduces the risk for loosing the template nucleic acid, which typically is present at low concentrations only. This is of particular importance, when performing RT-PCR. We therefore recommend the use of Uracil-DNA Glycosylase.
It has been used in LightCycler PCR assay to identify B. pertussis and B.parapertussis in nasopharyngeal swabs. It has also been used in quantitative PCR (qPCR) assay.

General Description

PCR Nucleotide MixPLUS is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
The incorporation of dUTP in place of dTTP allows the degradation of contaminating PCR products from former reactions with Uracil-DNA Glycosylase (UNG) to prevent carryover contamination from previous amplifications.

PCR NUCLEOTIDE MIX Preparation Products And Raw materials

Raw materials

Preparation Products

PCR NUCLEOTIDE MIX Suppliers

Global( 12)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
DNTP/DUTP MIX PCR NUCLEOTIDE MIX PCR NUCLEOTIDE MIXPLUS ADVANTAGE PCR DEOXYNUCLEOTIDE MIX PCR