In Situ Cell Death Detection Kit, TMR red

CAS No.
Chemical Name:
In Situ Cell Death Detection Kit, TMR red
Synonyms
In Situ Cell Death Detection Kit, TMR red
CBNumber:
CB24670069
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2022-08-15 17:41:15

In Situ Cell Death Detection Kit, TMR red Properties

storage temp. -20°C

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS08,GHS09
Signal word  Danger
Hazard statements  H350i-H411
Precautionary statements  P201-P273-P280-P308+P313-P391-P501
RIDADR  UN 1556 6.1 / PGIII

In Situ Cell Death Detection Kit, TMR red price More Price(1)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 12156792910 In Situ Cell Death Detection Kit, TMR red sufficient for ≤50?tests 50TESTS $643 2024-03-01 Buy
Product number Packaging Price Buy
12156792910 50TESTS $643 Buy

In Situ Cell Death Detection Kit, TMR red Chemical Properties,Uses,Production

Uses

Precise, fast, and simple technique for detecting and quantitating apoptotic DNA fragmentation at ?the single-cell level in cells and tissues with a red fluorescent label for fluorescence microscopy and flow cytometry.

General Description

Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2′-deoxy-uridine. The methods involve the separation of fragmented, low molecular weight DNA from unfragmented, high molecular weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population, or particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3′-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3′-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3′-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.

Sample material: Cells in suspension, cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.

Principle
The In Situ Cell Death Detection Kit, TMR red is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and TMR-dUTP. During this incubation period, TdT catalyzes the addition of TMR-dUTP at free 3′-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is visualized by flow cytometry and/or fluorescence microscopy.

In Situ Cell Death Detection Kit, TMR red Preparation Products And Raw materials

Raw materials

Preparation Products

In Situ Cell Death Detection Kit, TMR red Suppliers

Global( 5)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Shanghai Universal Biotech Co.,Ltd 18768175414 gaojun@univ-bio.com China 24998 58
In Situ Cell Death Detection Kit, TMR red