KAPA Taq PCR Kit with dNTPs

CAS No.
Chemical Name:
KAPA Taq PCR Kit with dNTPs
Synonyms
KAPA Taq PCR Kit with dNTPs
CBNumber:
CB24670100
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

KAPA Taq PCR Kit with dNTPs Chemical Properties,Uses,Production

Uses

KAPA Taq PCR Kit with dNTPs has been used in:

  • High throughput PCR
  • Amplification of low copy DNA templates
  • Multiplex PCR
  • Specific amplification of complex templates
  • RT-PCR
  • Quantitative polymerase chain reaction (qPCR)

General Description

KAPA Taq is supplied in a 2X ReadyMix? format containing all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers, while KAPA Taq Buffer B is a Tris-potassium chloride buffer, which allows convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. All KAPA Taq Buffers are 10X buffers, containing 15 mM MgCl2 (1.5 mM at 1X). KAPA Taq DNA Polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.

Biochem/physiol Actions

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart? DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Taq PCR Kit with dNTPs Preparation Products And Raw materials

Raw materials

Preparation Products

KAPA Taq PCR Kit with dNTPs Suppliers

Global( 1)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
KAPA Taq PCR Kit with dNTPs