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3-Indolebutyric acid

Plant Growth Regulator Physical and Chemical Properties Preparation Analytical method Toxicity Chemical Properties Uses
3-Indolebutyric acid
3-Indolebutyric acid structure
Chemical Name:
3-Indolebutyric acid
Molecular Formula:
Formula Weight:
MOL File:

3-Indolebutyric acid Properties

Melting point:
124-125.5 °C(lit.)
Boiling point:
341.55°C (rough estimate)
1.1255 (rough estimate)
refractive index 
1.5440 (estimate)
storage temp. 
Clear colorless to pale yellow
Water Solubility 
Soluble in water(0.25g/L).
Air Sensitive
Stable. Incompatible with strong oxidizing agents. Light sensitive.
CAS DataBase Reference
133-32-4(CAS DataBase Reference)
EPA Substance Registry System
Indole-3-butyric acid (133-32-4)
  • Risk and Safety Statements
Signal word  Danger
Hazard statements  H301-H315-H319-H335
Precautionary statements  P261-P301+P310-P305+P351+P338-P280a-P301+P310a-P405-P501a
Hazard Codes  T,Xi
Risk Statements  25-36/37/38
Safety Statements  26-36-45-38-36/37/39-28A
RIDADR  UN 2811 6.1/PG 3
WGK Germany  3
RTECS  NL5250000
Hazard Note  Irritant
HazardClass  6.1
PackingGroup  III
HS Code  29339990
Toxicity LD50 i.p. in mice: 100 mg/kg (Anderson)

3-Indolebutyric acid price More Price(16)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 45532 Indole-3-butyric acid PESTANAL , analytical standard 133-32-4 250mg $42.9 2021-03-22 Buy
Sigma-Aldrich 57310 Indole-3-butyric acid ≥99.0% (T) 133-32-4 5g-f $51.4 2020-08-18 Buy
TCI Chemical I0026 3-Indolebutyric Acid >98.0%(HPLC)(T) 133-32-4 5g $25 2021-03-22 Buy
TCI Chemical I0026 3-Indolebutyric Acid >98.0%(HPLC)(T) 133-32-4 25g $73 2021-03-22 Buy
Alfa Aesar A15260 Indole-3-butyric acid, 98% 133-32-4 5g $37.7 2020-06-24 Buy

3-Indolebutyric acid Chemical Properties,Uses,Production

Plant Growth Regulator

Plant growth regulators are artificially synthetic, physiologically active organic compound that is similar as plant hormones in regulating physiological processes of plants, controlling of plant growth and reproduction of organic compounds. Indole butyric acid (IBA) and naphthalene acetic acid (NAA) are two commonly used plant growth regulator in rooting cuttings with indole butyric acid working best because it has less operation in plants and is easy to be maintained in the vicinity of the site. Furthermore, using root pro-growth regulators in combination with other beneficial growth regulators can play an efficacy-enhancing role or synergistic role which enable a more satisfactory results for plants which are difficult get rooting. Indole butyric acid has a strong rooting-promotion effect but has the adventitious roots be thin and long while the rooting-promotion effect of naphthalene acetic acid (NAA) is less but coarse root. Therefore, we can usually get better results through the mixture of the two.
In 1928, Went (F. W. Went) had first successfully isolated this chemical messenger which causes the bending of the coleoptiles, named auxin. In 1934, Kogl (F.Kogl), etc has identified it as indole acetic acid. Accordingly, the indole acetic acid is often used as a synonym for auxin in custom. Shortly after recognizing the role of indole acetic acid on regulating the growth effect, scientists further found that indole propionic acid (IPA) and indole butyric acid (IBA) also has physiological effects similar to auxin. Later it was also found that naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,6-trichloro-benzoic acid and 4-amino-3,5,6-trichloro-pyridine carboxylic acids and their derivatives (including salts, esters, amides, such as sodium naphthalene acetic acid, 2,4-D butyl ester, naphthyl ethyl amide) all have the activity of auxin.
3-Indolebutyric acid is not easy to be oxidized in plants and has a poor conductivity. It has similar physiological effect as indole acetic acid. It can take effect in cell division and cell growth of plants but with a less significant effect compared with indole acetic acid is mainly used to promote rooting cuttings and effectively promote cell division cambium. It has a relative long maintaining time for its efficacy and can promote more but slender adventitious roots with better efficacy when being administered together with naphthalene acetic acid.
Indole-3-butyric acid can be applied to the cuttings and root cutting of chrysanthemum and other ornamental plants for facilitating the rooting at a concentration of 0.5~1.0mg/L. However, we should not be applied to the foliage of the plants. Degradation and Metabolism: it can subject to rapid degradation in the soil.
The above information is edited by the Chemicalbook of Dai Xiongfeng.

Physical and Chemical Properties

3-Indolebutyric acid: (molecule formula: C11H12O2N) has a molecular weight of 190.22. The pure product is white crystalline solid with the melting point being 124~125 ℃. Its industrial product is white to pale yellow crystals with a melting point being 121~124 ℃. It has special smell and is toxic! It is irritant with a vapor pressure being <10μPa at 60 ℃. It is difficult to be dissolved in water with the solubility in water at 20 ℃ being 0.25g/L. It is easily soluble in benzene and soluble in other organic solvents. Its solubility (g/100ml) is: benzene> 100; acetone, ethanol, ethyl ether: 3 to 10; chloroform: 1 to 10. 3-indolebutyric acid has a low toxicity to humans and animals and is stable to the acid while forming salt in the solution of the hydroxide and carbonate compounds of the alkali metal. It can be produced from indole via Grignard reaction or by adding nitrile for hydrolysis. In Agriculture, 3-indolebutyric acid can be used as plant growth-promoting agent that can promote root growth and fruit ripening with an efficacy being stronger than indole acetic acid. Indole butyric acid is an excellent growth regulator for promoting the cutting and rooting of the cherry rootstock. You can apply rapid high-concentration dip method or soaking for treatment of cutting slips. Rapid high-concentrations dip method is through dipping the base of the cutting slips in IBA solution of a concentration of 5 × 10-4~1 × 10-2 for 5 seconds and immediately inserting the cutting slip in the bed. This method has the advantage of short processing time, even medication and excellent efficacy. After the treatment, adding simazine to the insertion beds (20.375 grams per meter) can lead to a higher rate of rooting.


Laboratory put the mixture of indole, potassium hydroxide, and poly oxalic acid, dried tetrahydronaphthalene and γ-butyrolactone for heating under reflux to obtain the indole butyric acid. The reaction equation is as follows:

Indole, potassium hydroxide, poly oxalic acid and dried tetrahydronaphthalene were added to the reaction vessel. The mixture was stirred for half an hour at room temperature; add γ-butyrolactone for heating reflux for 4 hours. The water remaining in the reaction system is removed by the water segregator. After cooling, the solid was totally dissolved by water with the separated organic layer being acidified with hydrochloric acid for precipitating a large number of off-white solid. Use suction filtration, wash with water, and dry to give the crude indole butyric acid with the yield of the crude product being 92.6%. After recrystallisation but aqueous ethanol, you can obtain white flaky crystals that is indole-3-butyric acid with the mp being 121.5~123 ℃.

Reference: Daquan Wang (editor), “fine chemical production route” 2nd version, Beijing: Chemical Industry Press .1999 p. 260-261.

Analytical method

1. Product analysis: in the absolute ethanol, measure it at wavelength of 281nm through UV spectrophotometry (ACF Chemiefarma method).
2. Residue Analysis: use chloroform for extraction from the acid medium, and put its methanol solution together with petroleum ether (40~60 ℃) for shaking absorption, and further use high performance liquid chromatography for applying ultraviolet detection (ACF Chemiefarma France). Alternatively, you can also refer to the indole acetic acid residue analysis. First derive methyl or the trimethyl ester of silane, and then use gas chromatography for analysis.


It has low toxicity to humans and animals. Acute-oral rat: LD50: 5000mg/kg, mice 1760mg/kg. Carp LC50: 180mg/L (48h). It has low toxicity to bees.

Chemical Properties

The pure product is white crystals. m.p.124~125 ℃, vapor pressure <10 × 10-6Pa) (60 ℃). It is easily soluble in organic solvents but insoluble in water. It is stable to acid. The industrial product is white to pale yellow crystals with a melting point of 121~124 ℃.


Indole butyric acid (IBA) is a broad spectrum indole-class plant growth regulators and is a good rooting agent. It can promote the cuttings and rooting of herbaceous and woody ornamental plant. It can also be applied to the fruit setting of fruit as well as improving the fruit setting rate.

Chemical Properties

white to light yellow crystalline powder


Used to stimulate root formation of plant clippings. Suitable for plant cell culture tested.


To stimulate root formation of plant clippings.

Purification Methods

Recrystallise the acid from H2O. It is soluble in EtOH, Et2O and Me2CO but insoluble in CHCl3. [Bowman & Islip Chem Ind London 154 1971, Jackson & Manske J Am Chem Soc 52 5029 1930, Albaum & Kaiser Am J Bot 24 420 1937.] It has also been recrystallised from EtOH/water [James & Ware J Phys Chem 89 5450 1985]. Its UV has 278 and 320nm in isoPrOH [Elvidge Quart J Pharm Pharmacol 13 219 1940].max The methyl ester has m 73-74o (from *C6H6/pet ether) and b 230o/6mm [Bullock & Hand J Am Chem Soc 78 5854 1951]. [Beilstein 22 III/IV 1128, 22/3 V 140.]

3-Indolebutyric acid Preparation Products And Raw materials

Raw materials

Preparation Products

3-Indolebutyric acid Suppliers

Global( 593)Suppliers
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Shaanxi Dideu Medichem Co. Ltd
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Hubei Jusheng Technology Co.,Ltd.
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View Lastest Price from 3-Indolebutyric acid manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2021-06-03 3-Indolebutyric acid
US $92.00 / Kg/Bag 1KG 98% 1T Baoji Guokang Bio-Technology Co., Ltd.
2021-07-16 3-Indolebutyric acid
US $15.00 / KG 100g 99% 1000MT/Month Wuhan wingroup Pharmaceutical Co., Ltd
2021-07-08 3-Indolebutyric acid
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd

3-Indolebutyric acid Spectrum

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