Uses
REDExtract-N-Amp? seed PCR Kit has been used for DNA extraction.
General Description
The REDExtract-N-Amp Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
The kit comes with validated protocols to extract and amplify genomic DNA from corn, wheat, sunflower, cotton, soybean, sorghum, canola, and Arabidopsis seeds. Additional protocols are available. In a typical procedure, genomic DNA is extracted from a sample that has been ground and incubated in the Seed Preparation Solution and Extraction Solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the REDExtract-N-Amp PCR ReadyMix, included in the kit.
