SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)

Uses

SM(PEG)24 can be used as a crosslinker in:

• The study of chemical cross-linking of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and RNAses using the high-mass MALDI-MS technique.
• Preparation of protein G-liposomal nanovesicles applicable as sensors in the detection of gentamycin in the milk sample.
• Conjugation of catalase to streptavidin, which is helpful in the detection of analytes at very low concentrations using ELISA technique.

General Description

SM(PEG)n NHS- and maleimide-activated PEG compounds for crosslinking between primary amines (NH2) and sulfhydryl (SH) groups in proteins and other molecules. A complete set of compounds are offered, each having the same heterobifunctional structure (NHS-PEGn-Maleimide) but differing in the number of discrete ethylene glycol units ( n = 2, 4, 6, 8, 12 or 24). The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds