Membrane Scaffold Protein 1E3D1 D73C

CAS No.
Chemical Name:
Membrane Scaffold Protein 1E3D1 D73C
Synonyms
Membrane Scaffold Protein 1E3D1 D73C
CBNumber:
CB44670144
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

Membrane Scaffold Protein 1E3D1 D73C Properties

storage temp. -20°C

Membrane Scaffold Protein 1E3D1 D73C price

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich MSP09 Membrane Scaffold Protein 1E3D1 D73C recombinant, expressed in E. coli, Cysteine substituted at position 73 5MG $505 2023-06-20 Buy
Product number Packaging Price Buy
MSP09 5MG $505 Buy

Membrane Scaffold Protein 1E3D1 D73C Chemical Properties,Uses,Production

Uses

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

General Description

Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).

The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 D73C variant of MSP1 differs from MSP1 in the following facets:

  • It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1).
  • It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
  • It substitutes a cysteine (C) residue for an aspartic acid (D) residue in the Helix 2 (H2) portion of the protein, at position 73 of the original native A-1 sequence.
  • The initial histidine-tag is one amino acid shorter.

Membrane Scaffold Protein 1E3D1 D73C Preparation Products And Raw materials

Raw materials

Preparation Products

Membrane Scaffold Protein 1E3D1 D73C Suppliers

Global( 1)Suppliers
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Supplier Advantage
Sigma-Aldrich 80
Membrane Scaffold Protein 1E3D1 D73C