Digoxigenin-11-dUTP, alkali-stable
- CAS No.
- Chemical Name:
- Digoxigenin-11-dUTP, alkali-stable
- Synonyms
- Digoxigenin-11-dUTP, alkali-stable
- CBNumber:
- CB54669949
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Digoxigenin-11-dUTP, alkali-stable price More Price(3)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | DIUTPS-RO | Digoxigenin-11-dUTP, alkali-stable | 25μL | $336 | 2024-03-01 | Buy | |
Sigma-Aldrich | DIUTPS-RO | Digoxigenin-11-dUTP, alkali-stable | 125μL | $1290 | 2024-03-01 | Buy | |
Sigma-Aldrich | DIUTPS-RO | Digoxigenin-11-dUTP, alkali-stable | 5X125μL | $4930 | 2024-03-01 | Buy |
Digoxigenin-11-dUTP, alkali-stable Chemical Properties,Uses,Production
Uses
Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
General Description
Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Digoxigenin-11-dUTP, alkali-stable Preparation Products And Raw materials
Raw materials
Preparation Products
Digoxigenin-11-dUTP, alkali-stable Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
---|---|
Sigma-Aldrich | 80 |