KAPA Taq ReadyMix with dye

Uses

KAPA Taq ReadyMix^? with dye has been used in:

• high throughput PCR
• the amplification of low copy DNA templates
• multiplex PCR
• specific amplification of complex templates
• RT-PCR
• nested PCR (nPCR)
• amplifying exome 23 of anaplastic lymphoma kinase (ALK)
• polymerase chain reaction (PCR) amplification

General Description

KAPA Taq DNA Polymerase is a single-subunit Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. KAPA Taq ReadyMix^? (2X) is a ready-to-use cocktail containing all components for PCR, except primers and template. KAPA Taq ReadyMix is available with and without dye. The 2X ReadyMix with dye consists of two inert tracking dyes to permit direct loading of PCR products onto agarose gels for analysis by electrophoresis, without adding a DNA loading solution. KAPA Taq and KAPA Taq HotStart^? DNA Polymerase have 5′→3′ polymerase and 5→′3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Biochem/physiol Actions

KAPA Taq DNA Polymerase has 5′→3′ polymerase and 5′→3′ exonuclease activity. It does not elicit a 3′→5′ exonuclease (proofreading) activity. This enzyme system has an error rate of approximately 1 error per 2.2 X 105 nucleotides incorporated. PCR products created with KAPA Taq are A-tailed and are appropriate for cloning into TA cloning vectors.