Anti-Vinculin antibody, Mouse monoclonal

CAS No.
Chemical Name:
Anti-Vinculin antibody, Mouse monoclonal
Synonyms
Anti-Vinculin antibody, Mouse monoclonal
CBNumber:
CB64669805
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

Anti-Vinculin antibody, Mouse monoclonal Properties

storage temp. -20°C
form buffered aqueous solution

Anti-Vinculin antibody, Mouse monoclonal price More Price(4)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich V9264 Anti-Vinculin antibody, Mouse monoclonal clone hVIN-1, purified from hybridoma cell culture 25μL $167 2024-03-01 Buy
Sigma-Aldrich V9264 Anti-Vinculin antibody, Mouse monoclonal clone hVIN-1, purified from hybridoma cell culture 100μL $499 2024-03-01 Buy
Sigma-Aldrich V9264 Anti-Vinculin antibody, Mouse monoclonal clone hVIN-1, purified from hybridoma cell culture 200μL $644 2024-03-01 Buy
Sigma-Aldrich SAB4200080 Anti-Vinculin antibody, Mouse monoclonal clone V284, purified from hybridoma cell culture 200μL $601 2024-03-01 Buy
Product number Packaging Price Buy
V9264 25μL $167 Buy
V9264 100μL $499 Buy
V9264 200μL $644 Buy
SAB4200080 200μL $601 Buy

Anti-Vinculin antibody, Mouse monoclonal Chemical Properties,Uses,Production

Uses

Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting.

Uses

Monoclonal Anti-Vinculin is recommended to use in various immunochemical assays, including immunoblot (~120kDa), immunofluorescence, immunohistochemistry and immunoprecipitation.

Uses

Anti-Vinculin antibody, Mouse monoclonal has been used in

  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

General Description

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the VIN-11-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with smooth muscle vinculin from chicken gizzard. Vinculin, also known as metavinculin is highly conserved scaffolding protein localized to focal adhesions and adherens junctions. Vinculin is a 116 kDa protein with 1066 aminoacids. Vinculin comprises eight anti-parallel α-helical bundles organized into five distinct domains. Domains 1-3 are arranged into a tri-lobar head with a molecular mass of 95 kDa and diameter of 80? . The vinculin head binds many proteins including talin, IpaA, β-catenin, α-catenin and α-actinin. A 61 amino acid, proline-rich linker region (residues 837-878) connects the head and tail. The linker binds vasodilator-stimulated phosphoprotein (VASP), vinexin, ponsin and Arp2/3. The vinculin tail (amino acids 879-1066) is a 30 kDa helical bundle containing five helices connected by short loops (3-8 residues). The tail contains binding sites for vinculin head domain, paxillin, acidic phospholipids and actin. Within the cell vinculin exists either as open, active form or as a closed, auto-inhibited state. The vinculin gene is mapped to human chromosome 10q22.2. Metavinculin (150 kDa), a splice variant of vinculin, is co-expressed with vinculin in muscle tissues. These proteins are localized to the cell membrane, the I-band in the sarcomere and to the intercalated discs.

Biochem/physiol Actions

Vinculin is a cytoplasmic actin binding protein. It plays a key role in cell to cell and cell to matrix adhesion. It mediates adhesion by directly binding to F-actin and subsequently causing actin polymerization and binding of actin remodelling proteins. At high concentrations, Vinculin can undergo oligomerization and may be involved in the transmembrane assembly of adhesion plaques. Vinculin has been implicated in the control of adhesion, cell morphology and motility and muscle functions. Mutations in vinculin, affects the binding of vinculin to F-actin and impairs cell migration. It is a susceptible gene for dilated and hypertrophic cardiomyopathy. Mutations in vinculin may lead to sudden unexplained nocturnal death syndrome (SUNDS). In relation to cell adhesion and motility function, Vinculin is considered to have a crucial effect on tumor cells ability to invade tissues and hence to metastasize. Thus, it is suggested to be implicated in diagnosis of cancer progression and metastasis prognosis.

Anti-Vinculin antibody, Mouse monoclonal Preparation Products And Raw materials

Raw materials

Preparation Products

Anti-Vinculin antibody, Mouse monoclonal Suppliers

Global( 1)Suppliers
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Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
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Anti-Vinculin antibody, Mouse monoclonal