Titan One Tube RT-PCR System

Uses

Titan^? One Tube RT-PCR System has been used:

• to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
• for determining the presence or absence of RNA templates
• in cloning of rare messages without the need to construct cDNA libraries
• for quantifying the levels of gene expression

General Description

The Titan^? One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand^? High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:

• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.

Raw materials

Preparation Products