Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal

CAS No.
Chemical Name:
Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal
Synonyms
Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal
CBNumber:
CB64816054
Molecular Formula:
Molecular Weight:
0
MDL Number:
MOL File:
Mol file

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 04-808 Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal culture supernatant, clone 20.2, Upstate? 200μL $541 2023-01-07 Buy
Product number Packaging Price Buy
04-808 200μL $541 Buy

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal Chemical Properties,Uses,Production

Uses

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 04-808, Anti-dimethyl H3 (Arg2) at 1ug/mL (1:1000) dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition Analysis:
2 μM of histone H3 peptide containing dimethyl-Arg2 abolished detection of histone H3 by a previous lot of anti-dimethyl-Histone H3(Arg2) (1:4,000 dilution) in immunoblots of HeLa acid extracts. Some competition of signal was also seen with unmodified peptide containing Arg2.

Peptide Dot Blot Analysis:
A 1:4,000-1:16,000 dilution of a previous lot only detected histone H3 peptide containing dimethyl-Arg2. Peptides containing unmodi-fied Arg2, dimethyl-Arg17 or dimethyl-Arg26 were not detected.

Beadlyte Histone Peptide Assay:
1:500-1:48,000 dilutions of a previous lot detected histone H3 peptide containing dimethyl-Arg2. Cross-reactivity to peptides containing unmodified Arg2 or dimethylArg 17 or 26 was not seen.

General Description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 Arg2 methylation is a widespread silencing modification that inhibits histone H3 Lys4 trimethylation.

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal Preparation Products And Raw materials

Raw materials

Preparation Products

Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal Suppliers

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Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
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Anti-dimethyl-Histone H3 (Arg2), clone 20.2 Antibody, rabbit monoclonal