iPSC Induction Enhancer, Thiazovivin - Calbiochem

General Description

A cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 μM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461)and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.