MONOCLONAL ANTI-HUMAN IGG1 (FC SPECIFIC&

Uses

Monoclonal Anti-Human IgG1 antibody produced in mouse has been used in:

• direct hemagglutination(HA)
• hemagglutination inhibition(HAI) assays
• enzyme linked immunosorbent assay (ELISA)
• immunofluorometric assay (IFMA)
• immunofluorescence
• cell-based assays for the detection of IgG subclasses of acetylcholine receptor (AChR) antibodies

General Description

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 25, 6 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.

Biochem/physiol Actions

Human IgG1 is the predominant subclass of?in vivo?and?in vitro?produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes via Fc receptors (FcR). A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis.