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DAF-FM
- CAS No.
- 254109-20-1
- Chemical Name:
- DAF-FM
- Synonyms
- DAF-FM;DAF-FM solution;DAF-FM >=98% (HPLC);DIAMINOFLUORESCEIN-FM;PPRXFLLOWKZHMK-UHFFFAOYSA-N;DAF-FM - CAS 254109-20-1 - Calbiochem;4-Amino-5-methylamino-2′,7′-difluorescein;4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN;3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN;3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
- CBNumber:
- CB6679611
- Molecular Formula:
- C21H14F2N2O5
- Molecular Weight:
- 412.34
- MDL Number:
- MFCD03453541
- MOL File:
- Mol file
- MSDS File:
- SDS
Last updated:2023-05-04 15:13:37
| Melting point | 265 ℃ |
|---|---|
| storage temp. | -20°C |
| solubility | DMSO: >1mg/mL |
| form | Solid |
| pka | 8.19 ± 0.20, most acidic, temperature: 25 °C; 4.23 ± 0.20, most basic(at 25℃) |
| color | clear colorless to yellow |
| λmax | 487 nm, 495 nm |
| Biological Applications | Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264-P270-P301+P312-P330-P501 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| WGK Germany | 3 |
DAF-FM price More Price(4)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | D1821 | DAF-FM ≥98% (HPLC) | 254109-20-1 | 1mg | $590 | 2024-03-01 | Buy |
| Sigma-Aldrich | 251515-M | DAF-FM - CAS 254109-20-1 - Calbiochem A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. | 254109-20-1 | 251515-1MG | $999 | 2024-03-01 | Buy |
| Sigma-Aldrich | D2196 | DAF-FM solution 5 mM in DMSO, ≥97% (HPLC) | 254109-20-1 | 1mg | $737 | 2024-03-01 | Buy |
| Adipogen Life Sciences | CDX-A0523-M001 | DAF-FM solution ≥98%(HPLC) | 254109-20-1 | 1mg | $529 | 2021-12-16 | Buy |
DAF-FM Chemical Properties,Uses,Production
Uses
DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole.
Uses
DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.
Uses
DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
Biochem/physiol Actions
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
DAF-FM Preparation Products And Raw materials
Raw materials
Preparation Products
Global( 34)Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39916 | 58 |
| Alfa Chemistry | Info@alfa-chemistry.com | United States | 24072 | 58 | |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Alfa Chemistry | 1-516-6625404 | support@alfa-chemistry.com | United States | 9171 | 60 |
| Shanghai EFE Biological Technology Co., Ltd. | 021-65675885 18964387627 | info@efebio.com | China | 9715 | 58 |
| Energy Chemical | 021-58432009 400-005-6266 | marketing@energy-chemical.com | China | 44941 | 58 |
| Krain Pharmaceutical Technology CO.,Ltd | 18252089365 | Krain_kerun@163.com | China | 2618 | 58 |
| Henan Alpha Chemical Co., Ltd. | 0371-55013243 15324716602 | 2853979810@qq.com | China | 10010 | 58 |
| Supplier | Advantage |
|---|---|
| Chongqing Chemdad Co., Ltd | 58 |
| Alfa Chemistry | 58 |
| Sigma-Aldrich | 80 |
| Alfa Chemistry | 60 |
| Shanghai EFE Biological Technology Co., Ltd. | 58 |
| Energy Chemical | 58 |
| Krain Pharmaceutical Technology CO.,Ltd | 58 |
| Henan Alpha Chemical Co., Ltd. | 58 |
DAF-FM
DIAMINOFLUORESCEIN-FM
4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN
3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN
4-Amino-5-methylamino-2′,7′-difluorescein
DAF-FM solution
4-amino-5-methylamino-20,70-difluorodifluorofluorescein
PPRXFLLOWKZHMK-UHFFFAOYSA-N
DAF-FM - CAS 254109-20-1 - Calbiochem
3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-
DAF-FM >=98% (HPLC)
4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
4-amino-2',7'-difluoro-3',6'-dihydroxy-5-(methylamino)-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
254109-20-1
C21H14F2N2O5
Xanthene