Anti-PML antibody, Mouse monoclonal

Uses

Anti-PML antibody, Mouse monoclonal may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• indirect immunofluorescence

General Description

Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm.

Biochem/physiol Actions

A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport.