KAPA SYBRFAST One-Step
- CAS No.
- Chemical Name:
- KAPA SYBRFAST One-Step
- Synonyms
- KAPA SYBRFAST One-Step
- CBNumber:
- CB84670096
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS08 |
---|---|
Signal word | Warning |
Hazard statements | H371 |
Precautionary statements | P260-P264-P270-P308+P311-P405-P501 |
KAPA SYBRFAST One-Step Chemical Properties,Uses,Production
Uses
KAPA SYBR? FAST One-Step is suitable for:
- One-step quantitative reverse transcriptase PCR (qRT-PCR)
- Gene expression analysis
- Low-copy gene detection
- Microarray validation
- miRNA research
- Gene knockdown validation
Uses
KAPA SYBR? FAST One-Step
- Gene expression analysis
- Low-copy gene detection
- Microarray validationmiRNA research
- Gene knockdown validation
- Real-time quantitative reverse transcription-PCR (qRT-PCR)
- RNAi and miRNA research
General Description
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.
KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.
Biochem/physiol Actions
KAPA SYBR? FAST DNA Polymerase displays no enzymatic activity at ambient temperature. This prevents the formation of misprimed products and primer-dimers during reaction setup prior to the first denaturation step and results in high PCR specificity and accurate quantification. Moloney murine leukemia virus reverse transcriptase (M-MuLV-RT) has a high affinity for RNA and is optimized for cDNA synthesis at 42°C. The RNase Inhibitor safeguards against degradation of RNA target due to RNase contamination.
KAPA SYBRFAST One-Step Preparation Products And Raw materials
Raw materials
Preparation Products
KAPA SYBRFAST One-Step Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
---|---|
Sigma-Aldrich | 80 |