GENELUTE DIRECT MRNA MINIPREP KIT
- CAS No.
- Chemical Name:
- GENELUTE DIRECT MRNA MINIPREP KIT
- Synonyms
- Gen Elute;Blood Genomic DNA;GenElute? mRNA Kit;GenElute? Plasmid Kit;Bacterial Genomic DNA;GenElute? HP Plasmid Kit;Plant Genomic DNA Miniprep;GenElute? Mammalian RNA Kit;Mammalian Genomic DNA Miniprep;GenElute? HP Plasmid Miniprep Kit
- CBNumber:
- CB8509903
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
| Symbol(GHS) |     GHS02,GHS05,GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H226-H290-H302-H315-H319-H334-H336 |
| Precautionary statements | P210-P233-P234-P301+P312-P303+P361+P353-P305+P351+P338 |
| Hazard Codes | Xn,N,T,C |
| Risk Statements | 36/37/38-42-67-41-38-22-50/53-48/22-43-34-32-23/24/25-10-36/38-52/53-20/21/22-48/20 |
| Safety Statements | 23-24-26-36/37-39-61-45-36/37/39-36-22 |
| RIDADR | UN 3316 9 |
GENELUTE DIRECT MRNA MINIPREP KIT price More Price(15)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | RTN70 | GenElute™ Mammalian Total RNA Miniprep Kit sufficient for 70?purifications | 1kit | $417 | 2024-03-01 | Buy | |
| Sigma-Aldrich | RTN350 | GenElute™ Mammalian Total RNA Miniprep Kit sufficient for 350?purifications | 1kit | $1830 | 2024-03-01 | Buy | |
| Sigma-Aldrich | RTN10 | GenElute™ Mammalian Total RNA Miniprep Kit sufficient for 10?purifications | 1kit | $79 | 2024-03-01 | Buy | |
| Sigma-Aldrich | NA9604 | GenElute™ HP 96-Well Plasmid Miniprep Kit sufficient for 4 96-well plate purifications | 1kit | $1310 | 2024-03-01 | Buy | |
| Sigma-Aldrich | NA2120 | GenElute™ Bacterial Genomic DNA Kits sufficient for 350?purifications | 1kit | $920 | 2024-03-01 | Buy |
GENELUTE DIRECT MRNA MINIPREP KIT Chemical Properties,Uses,Production
Uses
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
General Description
Sigma′s GenElute Mammalian Total RNA Miniprep Kit provides a simple and convenient way to isolate total RNA from mammalian cells and tissues. Protocols are provided for cells, tissues, and fibrous tissues. These protocols differ in their cell lysis and disruption conditions. Once the RNA is bound to the GenElute Binding Column, the purification procedure is the same for all starting materials. For fibrous tissues the kit must be used with proteinase K (Cat. No. P4850) to ensure effective cell disruption. The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for cesium chloride gradients, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform. Cells or tissues are lysed and homogenized in a buffer containing guanidine thiocyanate to ensure thorough
denaturation of macromolecules and inactivation of RNases. Addition of ethanol causes RNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove contaminants, RNA is eluted in 50-100 μL of Elution Solution. Up to 150 μg of total RNA can be isolated in less than 30 minutes.
If all traces of DNA contamination must be eliminated, further treatment with DNase I is recommended. DNase I digestion can be performed while the RNA is bound to the GenElute Binding Column using the On-Column DNase I Digestion Set (DNASE10 and DNASE70). Alternatively, for more stringent removal of contaminating DNA, the final RNA preparation can be treated with Amplification Grade DNase I (AMPD1).
GENELUTE DIRECT MRNA MINIPREP KIT Preparation Products And Raw materials
Raw materials
Preparation Products
GENELUTE DIRECT MRNA MINIPREP KIT Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
| Supplier | Advantage |
|---|---|
| Sigma-Aldrich | 80 |