Uses
MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis. It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS3 (mass spectrometry) method for leucine/isoleucine discrimination.
General Description
MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.
Components:
Each vial contains various amounts of 14 isotopically labeled synthetic peptides dried via vacuum centrifugation. Each peptide is labeled with either a [13C6,15N2] Lysine (+8 Da), a [13C6,15N4] Arginine (+10 Da), or a [13C6,15N] Leucine (+7 Da).
