Anti-EEA1 antibody, Mouse monoclonal

Uses

Anti-EEA1 antibody, Mouse monoclonalhas been used in:

• immunoblotting
• immunoprecipitation
• cell fractionation
• indirect immunofluorescence
• immunohistochemistry

General Description

Monoclonal Anti-EEA1 (mouse IgG2a isotype) is derived from the hybridoma EEA1-N19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EEA1. Early Endosome Antigen 1 (EEA1) is a dimer. It comprises extensive coiled-coil regions, and at its C-terminus contains a cysteine-rich zinc-finger-like domain named FYVE domain.

Biochem/physiol Actions

Early Endosome Antigen 1 (EEA1) FYVE domain is involved in intracellular trafficking and is implicated in the specific localization of EEA1 to endosomes . Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rabaptin-5 (Rab5). The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns 3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Monoclonal Anti-EEA1 may be used as an early endosome marker.