Creatine Kinase (CK)

Uses

Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.

General Description

ATP:creatine N-phosphotransferase
Creatine kinase (CK) has a molar mass of 85kDa. It exists as a dimer in the cytosolic form and as a dimer and an octamer in the mitochondrial form. The cytosolic forms are named as MM, BB, and MB, where M and B correspond to muscle and brain, respectively. CK from rabbit muscle is a dimer containing two identical polypeptide chains of 380 amino acids. Each subunit has four cysteines, Cys73, Cys145, Cys253 and Cys282, but lacks inter- and intra-chain disulfide bonds.

Biochem/physiol Actions

Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.

Raw materials

Preparation Products