ddNTP set 化学特性,用途語,生産方法
使用
2′,3′-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase (
e.g., Klenow enzyme) and are therefore used for DNA sequencing according to Sanger (Sanger, F.
et al. (1977) PNAS USA
74, 5463).
Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T).
一般的な説明
The Dideoxynucleoside Triphosphate Set, Sequencing Grade, sodium salt solution, consists of dideoxynucleotides of very high purity (ddNTP HPLC, area% ≥98%; ddNDP HPLC, area% ≤1.5%), specifically manufactured and tested for sequencing reactions.
The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).
ddNTP set 上流と下流の製品情報
原材料
準備製品