ポドフィロトキシン 化学特性,用途語,生産方法
外観
白色〜ほとんど白色, 粉末
溶解性
エタノールに可溶アセトン及びエタノールに溶け、水にほとんど溶けない。
解説
C22H22O8(414.40)．ポドフィロトキシンは，メギ科Podophyllum peltatumなどの地下茎からβ-D-グルコシドとして得られる．融点114～118 ℃．[α]20D－132°(クロロホルム)．エタノールや有機溶媒に可溶，水に難溶．抗腫瘍作用を有するが，毒性が強く，皮膚に炎症を起こす．LD50 8.7 mg/kg(ラット，静注)．森北出版「化学辞典（第2版）
用途
ポドフィロトキシン（podophyllotoxin, PPT、ポドフィロックス）は、ポドフィルム属植物の根および根茎に含まれる毒性リグナンである。局所ゲル剤がコンジロックスの商品名で、一部のヒトパピローマウイルスが原因の外性器尖圭コンジローマの治療に用いられる。ポドフィロトキシンおよびその誘導体（エトポシド、テニポシド、エトポホス）は、瀉下薬、発疱薬、抗リウマチ薬、抗ウイルス薬、抗腫瘍剤と幅広く応用されている。これらの抗がん活性は精力的に研究されており、肺がん、リンパ腫、生殖器腫瘍などに対する治療に使用されている。
効能
抗悪性腫瘍薬, 抗ウイルス薬, 微小管重合阻害薬
説明
Podophyllotoxin (2,3-butyl-4-aromatic naphthene) is isolated from guijiu(Podophyllum) . There are two species as the main source of podophyllotoxin,
Podophyllum hexandrum Royle and Podophyllum peltatum .
Although podophyllotoxin has significant antitumor and antiviral activities, it
showed several toxicity and side effects. Podophyllotoxin derivatives, etoposide
(VP-16-213), Etopophos, amino sugar etoposide (NK6l1), and teniposide (VM26),
have been developed as anticancer drugs. They are used to treat small cell lung cancer, testicular cancer, acute leukemia, malignant lymphoma, etc. But podophyllotoxin derivatives are not free of toxicity. Besides the narrowing of the anticancer
spectrum and low water solubility, these drugs could induce severe myelosuppression, gastrointestinal side effects, etc.
Although the synthetic and biosynthetic pathways of podophyllotoxin have been
elucidated, it is still the most effective, economic, and fast way to extract podophyllotoxin from the plant.
化学的特性
off-white fine crystalline powder
物理的性質
Appearance: white needle crystal powder. Solubility: freely soluble in chloroform,
acetone, ethyl acetate, and benzene; soluble in ethanol and ethyl ether; and insoluble
in water. Melting point: after drying the melting point is 183–184°C. Specific optical rotation: 132.7°C (chloroform).
来歴
Podophyllotoxin was first found in the Podophyllum peltatum L.?The first time to
isolate podophyllotoxin from podophyllin was in 1880. In 1942, it was found that
venereal warts could be effectively treated by application of podophyllin.Subsequently, podophyllotoxin was reported to inhibit the growth of the tumor
through the inhibition of the microtubule formation. The chemical structure of
podophyllotoxin was elucidated in 1951.
In the 1960s, two main podophyllotoxin derivatives were synthesized, etoposide
and teniposide (VM-26) . In 1983, etoposide was approved by FDA.?Etoposide
and teniposide are used in frontline cancer therapy against various cancer types,
such as small cell lung cancer, testicular cancer, etc. In 1996, etoposide phosphate
analog (Etopophos) was launched in America. Etopophos is the prodrug of etoposide and can be rapidly absorbed and completely converted to the parent compound
in?vivo. In 1990, WHO recommended 0.5% podophyllotoxin as the first-line drug
for the treatment of condyloma acuminatum. Podophyllotoxin creams and gels are
nowadays widely used in clinical practice.
使用
Podophyllotoxin is a non-alkaloid toxin lignan extracted from the roots and rhizomes of Podophyllum species. It binds to topoisomerase II during the late S and early G2 stage, blocking tubulin polymerization and, thus, inhibiting mitosis. In addition to being used as a cathartic, purgative, antiviral agent, vesicant, and antihelminthic, podophyllotoxin is the starting material for the semi-synthesis of the anti-cancer drugs etoposide , teniposide , and etopophos.
定義
ChEBI: An organic heterotetracyclic compound that has a furonaphthodioxole skeleton bearing a 3,4,5-trimethoxyphenyl substituent. It is found in the roots and rhizomes of Podophyllum species and is used for the topical treatment of genital warts.
適応症
Podophyllotoxin (Podofilox) is available alone and as
the main cytotoxic ingredient in podophyllin (25%
podophyllum resin), a mixture of toxic chemicals derived
from May apple plants. The active ingredients inhibit
cell mitosis. The drugs are used to treat condylomata
acuminata. The most common toxic effects are
skin irritation and less commonly, ulceration. Systemic absorption of podophyllin can occur (especially if applied
to large, inflamed areas or mucosal surfaces), with
gastrointestinal, hematological, renal, and hepatotoxic
effects. In addition, seizures and peripheral neuropathy
have been reported.
薬理学
Antineoplastic and antiviral activities are the most pronounced pharmacological.
effects of podophyllotoxin . Podophyllotoxin shows a significant inhibitory
effect on the division and proliferation of epithelial cells infected by human papillomavirus (HPV), disrupts the cell cytoskeleton, and induces the necrosis and shedding of warts. It was shown that the antitumor effect of podophyllotoxin is associated
with the inhibition of microtubule assembly and the induction of apoptosis.
However, the antitumor effect of podophyllotoxin analogs, such as etoposide, teniposide, and Etopophos, is related to disparate mechanisms including the inhibition
of DNA topoisomerase II activity and the formation of stable nucleic acid-drugenzyme complex, which induce DNA double-strand or single-strand break and
eventually lead to cell death . It was also found that podophyllotoxin derivatives have immunosuppressive and anti-inflammatory effects.
臨床応用
Podophyllotoxin is a useful agent for the treatment of condyloma acuminatum .
Podophyllotoxin and its derivatives are also widely used in the treatment of cancer,
such as lymphomas and lung carcinoma. Because of the several toxicity of podophyllotoxin, for example, the irritation of skin and mucous membranes, combination therapies are used to treat condyloma acuminatum or cancer.
抗がん研究
Podophyllotoxin (PTOX) is an aryl-tetralin lignan and has been originallyisolated from Podophyllum peltatum L. (American podophyllum or Mayapple;family Podophyllaceae). Later, it is also isolated from several species like P.hexandrum Royle (Indian podophyllum) and P. pleianthum (Taiwanese podophyllum).PTOX has also been reported in other plants such as Linum spp., Callitrisspp., Juniperus spp., Thuja spp., Hyptis spp., Thymus spp., Teucrium spp., Nepetaspp., Dysosma spp., Diphylleia spp., and Jeffersoniana spp. (Ionkova 2007;Yousefzadi et al. 2010). PTOX shows strong cytotoxic activity against various cancercell lines. However, PTOX is too toxic for the treatment of neoplastic diseasesin humans; it is used as a precursor for chemical synthesis of semisynthetic antineoplasticdrugs, etoposide, Etopophos, and teniposide , which are successfullyused as antitumor agents (Holthuis 1988; Cragg and Newman 2005).Podophyllotoxin derivatives are used in the treatment of lymphomas, acute leukemia,and testicular, lung, ovarian, bladder, and brain cancer (Srivastava et al. 2005).Podophyllum spp. are the major source of PTOX, and their availability is limited innature, and some species are categorized as endangered. Moreover, the chemicalsynthesis of podophyllotoxin is an expensive process; therefore, biotechnologicalproduction of podophyllotoxin using plant cell and tissue cultures has been preferredby various research groups (Farkya et al. 2004).
純化方法
The toxin recrystallises form *C6H6 (with 0.5C6H6), EtOH/*C6H6, aqueous EtOH (with 1-1.5H2O, m 114-115o) and CH2Cl2/pentane. When dried at 100o/10mm it has m 183-184o. [UV: Stoll et al. Helv Chim Acta 37 1747 1954, IR: Schecler et al. J Org Chem 21 288 1956.] It is an inhibitor of microtubule assembly [Prasad et al. Biochemistry 25 739 1986]. [Beilstein 19/10 V 666.]
ポドフィロトキシン 上流と下流の製品情報
原材料
準備製品