KAPA Taq PCR Kit with dNTPs 化学特性,用途語,生産方法
使用
KAPA Taq PCR Kit with dNTPs has been used in:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
- Quantitative polymerase chain reaction (qPCR)
一般的な説明
KAPA Taq is supplied in a 2X ReadyMix
? format containing all the components required for PCR except primers and template. Simply use PCR-grade water to make up the required reaction volume. KAPA Taq Buffer A (and KAPA Taq Buffer with dye) are standard Tris-ammonium sulfate-based buffers, while KAPA Taq Buffer B is a Tris-potassium chloride buffer, which allows convenient direct analysis of the PCR product by agarose gel electrophoresis after cycling. All KAPA Taq Buffers are 10X buffers, containing 15 mM MgCl2 (1.5 mM at 1X). KAPA Taq DNA Polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.
Biochem/physiol Actions
KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart? DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Taq PCR Kit with dNTPs 上流と下流の製品情報
原材料
準備製品