GC-RICH PCR System

GC-RICH PCR System 価格 もっと(2)

メーカー	製品番号	製品説明	CAS番号	包装	価格	更新時間	購入
Sigma-Aldrich Japan	12140306001	sufficient for ≤50?reactions, pkg of 100?U, optimum reaction temp. 72 °C GC-RICH PCR System sufficient for ≤50?reactions, pkg of 100?U, optimum reaction temp. 72 °C		50REACTIONS	￥38100	2024-03-01	購入
Sigma-Aldrich Japan	12140306001	GC-RICH PCR System		12140306001	￥31600	2018-12-25	購入

GC-RICH PCR System 化学特性,用途語,生産方法

使用

The GC-RICH PCR System, a blend of Taq DNA polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superb performance – especially from problematic templates.
The GC-RICH PCR System may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA polymerase alone.

Advantages of the GC-Rich PCR System:

• Ease access to difficult templates, including GC-rich targets and repetitive sequences.
• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.

一般的な説明

The GC-RICH PCR System is composed of an enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR Reaction buffer in combination with the separately included GC-RICH resolution solution allows to amplify difficult templates like GC-rich targets very efficiently. TA cloning is recommended. The enzyme blend produces more blunt-ended fragments than Taq DNA Polymerase. The majority of products have single A overhangs. Use 2 U for a standard 50 μl PCR.

Contents

• GC-RICH Enzyme Mix , in storage buffer
• GC-RICH Reaction Buffer, 5x concentrated, with 7.5 mM MgCl2 and DMSO
• GC-RICH Resolution Solution, 5 M
• MgCl2 Stock Solution, 25 mM
• Water, PCR Grade

GC-RICH PCR System 上流と下流の製品情報

原材料

準備製品

GC-RICH PCR System 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

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• GC-RICH PCR System