Apoptotic DNA-Ladder Kit

Apoptotic DNA-Ladder Kit 価格 もっと(1)

メーカー	製品番号	製品説明	CAS番号	包装	価格	更新時間	購入
Sigma-Aldrich Japan	11835246001	Apoptotic DNA-Ladder Kit		11835246001	￥48400	2018-12-25	購入

Apoptotic DNA-Ladder Kit 化学特性,用途語,生産方法

使用

The Apoptotic DNA-Ladder Kit provides rapid isolation of DNA, which can be analyzed and characterized by gel electrophoresis for the determination of apoptotic cell death.

一般的な説明

This kit provides a way to isolate apoptotic DNA fragments for DNA ladder analysis. The purification method used is much faster than other DNA purification methods (e.g., phenol/chloroform extraction, DNA precipitation). Purified DNA can be mixed directly with gel loading buffer and analyzed on an agarose gel.

The study of cell death involves characterizing mortality as apoptotic or necrotic. Apoptosis can be characterized by:

• Prelytic, non-random fragmentation of DNA (“ladder” pattern after agarose-gel electrophoresis)1
• Formation of membrane-bound vesicles (or “apoptotic bodies”)
• Cell shrinkage due to a concentration of cytoplasm

Similarly, necrosis (or physiological cell death) is characterized by

• Random digestion of DNA (DNA smear after agarose-gel electrophoresis)
• Swollen organelles and cells, resulting from loss of membrane integrity and cell lysis
• Postlytic DNA fragmentation

As these differences indicate, the classification of cell death can be accomplished through observing cell morphology, or less subjectively, by analyzing genomic DNA. Resolving the DNA on a gel provides a quick and documentable form of data to differentiate between apoptosis and necrosis.

Apoptotic DNA-Ladder Kit 上流と下流の製品情報

原材料

準備製品

Apoptotic DNA-Ladder Kit 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

---

• Apoptotic DNA-Ladder Kit