PCR Buffer Without MgCl2, 10x concentrated 化学特性,用途語,生産方法
使用
The PCR buffer without MgCl
2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).
一般的な説明
The PCR buffer without MgCl
2, in combination with the MgCl
2 stock solution, is used for the individual adjustment of the Mg
2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl
2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg
2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl
2 stock solution.
PCR Buffer Without MgCl2, 10x concentrated 上流と下流の製品情報
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