PCR Buffer Without MgCl2, 10x concentrated C화학적 특성, 용도, 생산
용도
The PCR buffer without MgCl
2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).
일반 설명
The PCR buffer without MgCl
2, in combination with the MgCl
2 stock solution, is used for the individual adjustment of the Mg
2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl
2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg
2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl
2 stock solution.
PCR Buffer Without MgCl2, 10x concentrated 준비 용품 및 원자재
원자재
준비 용품
