3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate 価格

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3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate 化学特性,用途語,生産方法

使用

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate is a zwitterionic detergent for membrane protein solubilization.

純化方法

Triturate this zwitterionic detergent (~50g) with MeOH (200ml), then triturate it with Me2CO (500mL), collect it by vacuum filtration and dry it thoroughly at ~20o. Recrystallise it at 0o from absolute MeOH followed by drying in vacuo to constant weight. It should give one spot with RF 0.32 by TLC on silica gel G in 95% MeOH/5% NH4OH and visualized with iodine vapour; the tertiary amine precursor has RF 0.40. It has low UV absorption (1% in H2O: A280nm is 0.029 and A260nm is 0.035), and the CMC (critical micellar concentration) is 8mM. It is soluble in H2O, MeOH, Me2SO but insoluble in Me2CO, MeCN, hexane, toluene and xylene. [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate betaine) [82473-24-3] M 630.89, m 184-196o, [ ] D +19o (c 2, H2O), pKEst (1) ~<2 (acidic), pKEst(2) ~9 (basic). Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate 上流と下流の製品情報

原材料

準備製品

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate 生産企業

名前	電話番号	電子メール	国籍	製品カタログ	優位度
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49391	58
Aladdin Scientific	+1-+1(833)-552-7181	sales@aladdinsci.com	United States	57511	58
Sinopharm Chemical Reagent Co,Ltd.	86-21-63210123	sj_scrc@sinopharm.com	China	9823	79
Energy Chemical	021-58432009 400-005-6266	marketing@energy-chemical.com	China	44941	58
Shanghai Aladdin Biochemical Technology Co.,Ltd.	+86-18521732826	market@aladdin-e.com	China	48467	58

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• 313223-04-0
• 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO)-&
• 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate monohydrate