KAPA Taq EXtra HotStart? ReadyMix? has been used for:
PCR of amplicons to be analyzed by agarose gel electrophoresis
PCR of short and medium length targets (<5 kb)
Long-range PCR (5 – 15 kb)
PCR with limiting amounts of template DNA
일반 설명
KAPA Taq EXtra HotStart? ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 μL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.