Ribonuclease H (RNase H)

Ribonuclease H (RNase H) 구조식 이미지
카스 번호:
상품명:
Ribonuclease H (RNase H)
동의어(영문):
Ribonuclease H (RNase H)
CBNumber:
CB24670579
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

Ribonuclease H (RNase H) 속성

물리적 상태
액체

안전

Ribonuclease H (RNase H) C화학적 특성, 용도, 생산

용도

Ribonuclease H (RNase H) has been used for:
  • In vivo RNA-primed initiation of DNA synthesis
  • Elimination of mRNA during second-strand cDNA synthesis
  • Site-specific cleavage of RNA
  • Detection of RNA:DNA regions in double-stranded DNA of natural origin
  • Removal of poly (A) sequences of mRNA if oligo (dT) is present
  • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

일반 설명

Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.

Biochem/physiol Actions

Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication.

Ribonuclease H (RNase H) 준비 용품 및 원자재

원자재

준비 용품


Ribonuclease H (RNase H) 공급 업체

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