ANTI-PROTEIN C AFFINITY MATRIX

ANTI-PROTEIN C AFFINITY MATRIX 구조식 이미지
카스 번호:
상품명:
ANTI-PROTEIN C AFFINITY MATRIX
동의어(영문):
ANTI-PROTEIN C AFFINITY MATRIX
CBNumber:
CB2491488
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

ANTI-PROTEIN C AFFINITY MATRIX 속성

저장 조건
2-8°C
물리적 상태
슬러리

안전

ANTI-PROTEIN C AFFINITY MATRIX C화학적 특성, 용도, 생산

용도

Anti-Protein C Affinity Matrix is used for:
  • Immunoprecipitation of Protein C-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of Protein C-tagged proteins from crude protein extracts

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-Protein C antibody
  • Silver staining (or similar protein stain)

일반 설명

Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Anti-Protein C mouse monoclonal antibody (clone HPC4) binds specifically to an epitope sequence spanning the thrombin cleavage site of protein C and is immobilized. Anti-Protein C recognizes the 12-amino acid sequence (EDQVDPRLIDGK), which encodes residues 6 through 17 of the heavy chain of Protein C. The formation of the Anti-Protein C/protein C epitope complex is dependent on the presence of calcium ions. In the presence of Ca2+, the antibody binds with high affinity and specificity to this sequence in native human Protein C or in proteins tagged with this epitope. This efficient binding within the recombinant fusion protein occurs regardless of the site of incorporation of the epitope tag (i.e., N terminus, C terminus, or within the reading frame). This unique antibody is especially well suited for purification of recombinant fusion proteins tagged with the protein C epitope.

  • Insertion of the protein C tag does not introduce a new metal-ion binding site. The antibody contains the Ca2+ binding site.
  • Protein C tag can be integrated either at the N-terminus, C-terminus or internally without any change in antibody specificity.
  • Rapid immunoaffinity purification under non-denaturing conditions using economical calcium chelating agent (e.g., EDTA) or alternatively a specific protein C-tag peptide.

Monoclonal mouse antibody Anti-Protein C (clone HPC4) is covalently coupled to agarose beads. In the coupling reaction 4mg of antibody is reacted per 1ml of beads.

ANTI-PROTEIN C AFFINITY MATRIX 준비 용품 및 원자재

원자재

준비 용품


ANTI-PROTEIN C AFFINITY MATRIX 공급 업체

글로벌( 10)공급 업체
공급자 전화 이메일 국가 제품 수 이점
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

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