High Pure PCR Product Purification Kit

High Pure PCR Product Purification Kit 속성

High Pure PCR Product Purification Kit C화학적 특성, 용도, 생산

용도

The High Pure PCR Product Purification Kit is designed for the preparation of concentrated, purified DNA, and can be used directly for most molecular biology applications:

• Labeling
• Sequencing
• Cloning
• Restriction enzyme digests
• Alkaline phosphatase treatment
• Kinase reactions

The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications.

The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 μl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR.

일반 설명

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.

Capacity: The High Pure SpinFilter Tubes hold up to 700 μl sample volume.
Volume: 100 μl
Typical samples include:

• Products from amplification or cDNA synthesis reactions
• Enzymatically treated DNA
• DNA from agarose slices
• Dilute nucleic acid solutions
• RNA from transcription reactions

High Pure PCR Product Purification Kit 준비 용품 및 원자재

원자재

준비 용품

High Pure PCR Product Purification Kit 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80