MgCl2 Stock Solution

MgCl2 Stock Solution 구조식 이미지
카스 번호:
상품명:
MgCl2 Stock Solution
동의어(영문):
MgCl2 Stock Solution
CBNumber:
CB64670148
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

MgCl2 Stock Solution 속성

저장 조건
-20°C
물리적 상태
액체

안전

MgCl2 Stock Solution C화학적 특성, 용도, 생산

용도

The combination of PCR Buffer without MgCl2 and the MgCl2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).

일반 설명

The MgCl2 Stock Solution is used for the individual adjustment of the Mg2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl2. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and the yield of the PCR reaction.

Biochem/physiol Actions

Magnesium (Mg2+) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR). It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.

MgCl2 Stock Solution 준비 용품 및 원자재

원자재

준비 용품


MgCl2 Stock Solution 공급 업체

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