The FastStart TaqMan? Probe Master has been used in:
quantitative, real-time DNA-detection assays
qPCR and two-step reverse transcription (RT)-qPCR
in the hydrolysis probe detection format
일반 설명
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
