HEPES manufacturers and suppliers

HEPES suppliers

Properties

Melting point:: 234-238 °C

Boiling point:: 408℃[at 101 325 Pa]

bulk density: 560kg/m3

Density: 1.07 g/mL at 20 °C

vapor pressure: 0Pa at 25℃

refractive index: n20/D 1.339

storage temp.: 2-8°C

solubility: H₂O: 1 M at 20 °C, clear, colorless

form: powder

color: White

PH: 5.0-6.5 (1Maqueous solution)

pka: 7.5(at 25℃)

PH Range: 7.0 - 7.6

biological source: synthetic

Water Solubility: Soluble

λmax: λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

Merck: 14,4654

BRN: 883043

Stability:: Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.

InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N

LogP: -3.85 at 20℃

CAS DataBase Reference: 7365-45-9(CAS DataBase Reference)

EPA Substance Registry System: 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

Absorption: ≤0.05 at 290 in H2O at 33%

Safety Information

Symbol(GHS): GHS07

Signal word: Warning

Hazard statements: H302-H315-H319-H335

Precautionary statements: P261-P271-P280

Hazard Codes: Xi

Risk Statements: 36/37/38

Safety Statements: 24/25-22-36-26

WGK Germany: 1

RTECS: TL6809000

TSCA: Yes

HS Code: 29335995

Use

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

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