7-Amino-4-chloromethylcoumarin

Properties

Safety Information

Symbol(GHS):
Signal word:	Warning
Hazard statements:	Code Hazard statements Hazard class Category Signal word Pictogram P-Codes H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning P264, P270, P301+P312, P330, P501 H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362 H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning
Precautionary statements:	P261 Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Description

CytoTracer Blue CMAC is a blue fluorescent dye for labeling and long-term tracing of living cells. In working concentrations, the dye has little cytotoxicity and minimal effects on the proliferative ability or biology of the cell. It can be used to analyze cell proliferation, viability, localization, and motility for in vivo and in vitro assays.

CytoTracer Blue CMAC is a cell-permeant stain that converts into cell-impermeant reaction products inside the cell. During proliferation, the label is transferred to daughter cells, but not adjacent cells in the population, and labeled cells keep the fluorescence for at least 72 hours or through three to six cell generations.

CytoTracer Blue CMAC contains a chloromethyl group that reacts with thiol groups of internal cell components, utilizing a glutathione S-transferase-mediated reaction. This allows the stain to remain in cells after fixation and permeabilization steps and be used for subsequent immunofluorescence applications.