Citrullinated Myelin Basic Protein Polyclonal Antibody

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Description

Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.1 MBP contains 19 arginine residues that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.1,2 The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.1,3 The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.1 citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.1,3 Cayman’s Citrullinated Myelin Basic Protein Polyclonal Antibody can be used for Western blot and ELISA applications. The antibody recognizes citMBP at 21 kDa from human samples, and was generated using a peptide immunogen containing citrullines at R122 and R130 (human myelin basic protein sequence, isoform 5).WARNING This product is not for human or veterinary use.