| 71-67-0 Basic information More.. |
Product Name: | BROMOSULFALEIN | Synonyms: | bromsulphthalein;brom-tetragnost;bromthalein;bsfsimes;bsp;bspsodium;cbsp;disodiumbromosulfophthalein | CAS: | 71-67-0 | MF: | C20H11Br4NaO10S2 | MW: | 818.02 | EINECS: | 200-761-0 | Mol File: | 71-67-0.mol | |
Use
Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]
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71-67-0
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