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| | Disitertide Basic information |
| Product Name: | Disitertide | | Synonyms: | disitertide;L-Threonyl-L-seryl-L-leucyl-L-alpha-aspartyl-L-alanyl-L-seryl-L-isoleucyl-L-isoleucyl-L-tryptophyl-L-alanyl-L-methionyl-L-methionyl-L-glutaminyl-L-asparagine;P144 Peptide;P144;P144 PEPTIDE;TSLDASIIWAMMQN;DISITERTIDE, >98%;Disitertide,P144,P144 Peptide, >98%;L-Asparagine, L-threonyl-L-seryl-L-leucyl-L-α-aspartyl-L-alanyl-L-seryl-L-isoleucyl-L-isoleucyl-L-tryptophyl-L-alanyl-L-methionyl-L-methionyl-L-glutaminyl- | | CAS: | 272105-42-7 | | MF: | C68H109N17O22S2 | | MW: | 1580.84 | | EINECS: | | | Product Categories: | | | Mol File: | 272105-42-7.mol |  |
| | Disitertide Chemical Properties |
| Boiling point | 1968.7±65.0 °C(Predicted) | | density | 1.327 | | storage temp. | Store at -20°C | | solubility | DMSO : ≥ 200 mg/mL (126.52 mM);Water : < 0.1 mg/mL (insoluble) | | pka | 3.15±0.10(Predicted) | | InChIKey | IUYPEUHIWDMJLM-INGCBPSVNA-N |
| | Disitertide Usage And Synthesis |
| Description | Disitertide is an inhibitor of TGF-β1. It prevents TGF-β1-dependent inhibition of MV1Lu proliferation in vitro and markedly reduces binding of TGF-β1 to its receptors. Disitertide blocks TGF-β1-dependent stimulation of a reporter gene under the control of human α2(I) collagen promoter. Treatment with Disitertide at low doses causes a significant inhibition of HSC activation and a marked reduction in liver fibrosis in a rat model of chronic exposure to CCl4. A dose as low as 70 μg of the 15-mer peptide Disitertide, given to rats (300 g body weight) on alternate days, decreases fibrogenesis significantly during chronic exposure to CCl4. | | Biological Activity | Disitertide (P144) TFA is a polypeptide inhibitor of transforming growth factor TGFβ1, specifically blocking its interaction with the receptor. Disitertide (P144) is also an inhibitor of PI3K and an inducer of apoptosis. | | in vitro | Disitertide (100 μg/mL) suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells.
Disitertide (TGF-β1 inhibitor) abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance.
Disitertide (P144, 10 μg/mL to 200 μg/mL) affects proliferation,
Western Blot Analysis | Cell Line: | Mouse embryo osteoblast precursor MC3T3-E1 cells. | | Concentration: | | Concentration: | 100 μg/mL. | | Incubation Time: | 4 h. | | Result: | Significantly suppressed the protein expression levels of PI3K and p-Akt, and induced the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group. | | | in vivo | Disitertide (Topical application, 300 μg/mL) may promote scar maturation and clinical improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment.
| Animal Model: | Human hypertrophic scars were implanted in 60 nude mice.
| | Dosage: | 300 μg/mL was added the Lipogel. | | Administration: | Topical application daily administered. | | Result: | Successful shedding was achieved in 83,3% of the xenografts. | | | storage | Store at -20°C |
| | Disitertide Preparation Products And Raw materials |
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