Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Anti-Fluorescein-POD, Fab fragments Purity:sheep Yes Package:1EA Remarks:11426346910
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| Anti-Fluorescein-POD, Fab fragments Basic information |
| Anti-Fluorescein-POD, Fab fragments Chemical Properties |
storage temp. | 2-8°C | form | lyophilized (stabilized) |
| Anti-Fluorescein-POD, Fab fragments Usage And Synthesis |
Uses | Anti-Fluorescein-POD, Fab fragments are used for detection of fluorescein-labeled compounds in:
- Dot blot
- ELISA (enzyme-linked immunosorbent assay)
- Immunohistocytochemistry
- In situ hybridization
- Southern blot
- Western blot
| General Description | Fab fragments from polyclonal anti-fluorescein antibodies, conjugated to horseradish peroxidase. The reagent is an anti-fluorescein antibody, Fab fragments from sheep, conjugated with horse-radishperoxidase (POD). After immunization with fluorescein the sheep IgG (immunoglobulin G) was purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated with POD and stabilized in 60mM Tris-Hepes-buffer, 0.4% bovine immunoglobulin (w/v), 0.2% Germall II (w/v), pH 7.2. |
| Anti-Fluorescein-POD, Fab fragments Preparation Products And Raw materials |
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